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Relationship with Metalloproteinase 13, E-cadherin, Homocysteine and Co Factors Folic Acid and Vitamin B12 in Patients Diagnosed with Prostate Cancer

[ Vol. 17 , Issue. 1 ]

Author(s):

Thaís M. Gascón*, Beatriz G. Lourenço, Edimar C. Pereira, Beatriz A. da Costa Aguiar, Glaucia L. Veiga, Flavia de Sousa Gehrke, Fernando Adami and Fernando L.A. Fonseca   Pages 33 - 41 ( 9 )

Abstract:


Introduction: Prostate cancer (Pc) is the most frequent neoplasia in men and the second cause of death in Brazil.

Objective: To analyze the interactions and biologicals responses of Pc oxidative stress and prostatespecific antigen (PSA), E-cadherin and MMP-13. Demonstrate whether the increase of the amount of the form of E-cadherin found in the plasma of Pc patients, correlates with decrease of the PSA.

Methods: Samples were obtained through peripheral venipuncture to analyse parameters of biomarkers pc as PSA, E-cadherin, MMP-13, Homocysteine, Folic acid, Vitamin B12, Testosterone T and free following the patients diagnosis, 3 and 6 months during their treatment to analyze the biological responses of Pc oxidative stress.

Results: The analysis was performed by using immunoenzymatic assay. Statistical data processed through Excel in Windows Vista and analyzed through the Shapiro-wilk Test, ANOVA, and Spearman Test. An increase in the concentration of E-cadherin (p = 0.02), as a decrease in concentration of PSA (p < 0.001), total testosterone (p < 0.001) and free testosterone (p = 0.02) was observed during the treatment period without significant alterations in the remaining markers for either of the periods.

Discussion: It was found that during treatment of men diagnosed with pc that there was an an increase in the concentration of plasmatic E-cadherin, which was negatively correlated with the concentrations of folic acid (-0,03 (0,87) rs (p). It was observed that the levels of hcy are positively correlated with concentrations of total testosterone and a negative correlation. Vitamins B12 remained within the parameters of normality during the entire study.

Conclusion: P.S.A levels were free and total testosterone levels decreased. In this way, monitoring the folic acid, E-cadherin dosages of patients during the treatment phases can effectively complement in the face of remission, since it would be a way of preventing abnormal cell replications, with a clinical view prudent so that the cell methylation cycle is not affected.

Keywords:

Prostate cancer, metalloproteinase-13, E-cadherin, homocysteine, biomarkers, prostate-specific antigen.

Affiliation:

Laboratorio de Analises Clinicas, Faculdade de Medicina da Fundacao ABC, Santo Andre, SP, Estudante de Bioquimica e Biologia Molecular da Universidade British Columbia, Kelowna, Departamento de Ciencias Farmaceuticas, Universidade Federal de Sao Paulo, Diadema, SP, Laboratorio de Biologia Molecular, Faculdade de Medicina da Fundacao ABC, Santo Andre, SP, Laboratorio de Biologia Molecular, Faculdade de Medicina da Fundacao ABC, Santo Andre, SP, Laboratorio de Biologia Molecular e Parasitologia, Faculdade de Medicina da Fundacao ABC, Santo Andre, SP, Laboratorio de Estatistica e Epidemiologia, Faculdade de Medicina da Fundacao ABC, Santo Andre, SP, Laboratorio de Analises Clinicas, Faculdade de Medicina da Fundacao ABC, Santo Andre, SP

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