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High Antioxidant Capacities and Anti-inflammatory Effects of Hammada elegans Botsch. Extracts: An in vitro Assessment

[ Vol. 15 , Issue. 1 ]

Author(s):

Bensafiddine Feriha, Asseli Ibrahim, Mahfoudi Reguia, Djeridane Amar* and Yousfi Mohamed   Pages 54 - 67 ( 14 )

Abstract:


Introduction: The Algerian traditional medicines are supplied with the use of plants for the antiinflammatory effect treatment. The reasons for this were that pure compounds obtained also effective in reducing the toxicities of toxic agents or other drugs.

Methods: In this study, we go to explore the phytochemical composition and the phenolic content by indirect method, to evaluate the antioxidants and the anti-inflammatory capacities of twelve extracts from three plants.

Results: The total phenolic content, ranged from 0.168±0.020 to 4.166±0.124 mg per gram of dry weight. Phytochemical screening revealed that, tannins, C-heterosides, O-reduced heterosides and reducing compounds are the most common chemical groups. The highest antiradical activity was achieved with methanolic extract of Hammada elegans (EC50=0.551±0.171mg/mL). However, the acetonic extract of Hammada elegans represents the most important reducing activity (EC50=0.747±0.004mg/mL). Moreover, this extract also displays the highest chelating ferrous ions effect (EC50=5.749±0.009 mg/mL). While, the hydromethanolic extract of Cleome arabica had the best antilipoperoxidative effect (EC50=0.031±0.000mg/mL). Furthermore, all extracts inhibit the activity of lipooxygenase and cyclooxygenase with IC50 values less than19.210±0.297 mg/mL. Therefore, the acetonic extract of Hammada elegans appears to be twice as large, than that of standard inhibitors.

Conclusion: the fractionation of the acetonic extract of Hammada elegans, have given a potent bioactive compound which seems to have potential therapeutic possibilities for the prevention the inflammatory effects.

Keywords:

Anti-inflammatory effect, antioxidant capacity, CPA, Hammada elegans, isolation, phytochemical screening.

Affiliation:

Laboratoire des sciences fondamentales, Université Amar Telidji. PB 37G, 03000, Laghouat, Laboratoire des sciences fondamentales, Université Amar Telidji. PB 37G, 03000, Laghouat, Laboratoire des sciences fondamentales, Université Amar Telidji. PB 37G, 03000, Laghouat, Laboratoire des sciences fondamentales, Université Amar Telidji. PB 37G, 03000, Laghouat, Laboratoire des sciences fondamentales, Université Amar Telidji. PB 37G, 03000, Laghouat



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