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Screening of Actinomycetes For α-amylase Inhibitors Production

[ Vol. 15 , Issue. 1 ]

Author(s):

Shivabai Chandwad* and Sudhakar Gutte   Pages 40 - 44 ( 5 )

Abstract:


Background: Diabetes mellitus is the most common and fastest growing disease in the world. One of the therapy to treat diabetes is inhibition of α-amylase activity by inhibitors from microbial and plant source. Actinomycetes are potential sources of enzyme inhibitors, drugs, amino acids, vitamins etc.

Objective: Our work mainly highlights on the isolation of actinomycetes from soil samples of different habitat and screening for α -amylase inhibitors.

Methods: Actinomycetes are isolated from soil samples of different habitat by different methods, these include a variety of pre-treatment of soil samples in combination with appropriate supplement medium with selective antibacterial agents. Isolated actinomycetes grown in fermentation condition and metabolites are extracted with Isopropyl alcohol and concentrated to get solid material. Extract of each isolates tested for α -amylase inhibition using starch Iodine plate method and DNS- spectroscopic method.

Results: Total 110 actinomycetes strains are isolated from various sources. Among 110 extracts of actinomycetes, eight extracts have shown positive results for α-amylase inhibition in starch Iodine plate assay method. Extracts selected from primary results used for the confirmation of inhibitory activity using DNS- spectroscopic method. Out of eight extract, six extracts were shown Porcine pancreatic α-amylase inhibitory activity range from 40-86%. The actinomycetes strains that produce α -amylase inhibitory activity are A-24, A-29, B-5, B-18, C-15 and D-24.

Conclusion: These results show that actinomycetes are potential source for α -amylase inhibitors, which may lead to valuable novel drugs for diabetic treatment.

Keywords:

Actinomycetes, amylase, diabetics, inhibitors.

Affiliation:

Department of Microbiology, PVP College, Patoda, Dist-Beed. Pin 414204.MH, Department of Microbiology, PVP College, Patoda, Dist-Beed. Pin 414204.MH



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