Youcef Boubrima*, Gouzi Hicham, Pierre Stocker, Abdelkarim Kameli and Mohamed Yousfi* Pages 196 - 202 ( 7 )
Background: Diabetes mellitus is the most common disease in the world. One of the approaches therapy used to treat diabetes is inhibition of α -glucosidase activity by natural inhibitors from the plant.
Objective: Our work comes to highlight, in vitro, the effect of phenolic extracts of some Algerian Saharan Atlas medicinal plants on the activity of α-glucosidase.
Methods: The ethyl acetate extracts were prepared from hydro-methanolic (80:20) extracts of studied plants. The Folin-Ciocalteu method was used to determine the total phenolic content of the extracts. The α-glucosidase activity was determined by a microplate spectrophotometer at 405 nm using paranitrophenol α-D-glucopyranose as substrate.
Results: Among the plants tested Arthrophytum scoparium, Helianthemum lippii, Equisetum arvense and Cistus villosus exhibited a strong α-glucosidase inhibitory activity. According to IC50 values, Arthrophytum scoparium exerted the highest inhibitory activity against α-glucosidase (IC50= 5.02 µM) while a weak inhibitory activity was observed with Equisetum arvense (IC50 =21.88 µM). The kinetic study revealed a noncompetitive type inhibition for Arthrophytum scoparium (KI = 4.16 µM) and Helianthemum lippii (KI = 1.46 µM) extracts whereas a full mixed type inhibition (KI = 18.17 µM; KIS = 3.98 µM) and competitive type inhibition (KI = 25 µM) were observed for Cistus villosus and Equisetum arvense, respectively.
Conclusion: The results suggest that these plants represent a potential source to develop new antidiabetic drugs for controlling postprandial blood sugar.
Atlas algerian plants, diabetes mellitus, inhibition, kinetic, phenolic extracts, α-glucosidase.
Laboratoire des Sciences Fondamentales, Faculte des Sciences, Universite Amar Telidji, BP 37G Route de Ghardaia 03000, Laghouat, Laboratoire de Chimie Organique, Substances Naturelles et Analyse (COSNA), Departement de Chimie, Faculte des Sciences, Universite Abou Bekr Belkaid, Tlemcen, CNRS, Institut de Chimie Radicalaire UMR 7273, Equipe Sondes Moleculaires en Biologie et Stress Oxydant, Marseille, Laboratoire d'ethnobotanique et de substances naturelles, Ecole Normale Superieure BP 92, Kouba-Alger, Laboratoire des Sciences Fondamentales, Faculte des Sciences, Universite Amar Telidji, BP 37G Route de Ghardaia 03000, Laghouat