Wissam Zam*, Aziz Khaddour and Hala Suliman Pages 146 - 151 ( 6 )
Background: It has been considered that Helicobacter pylori, a bacterium associated with a number of important upper gastrointestinal conditions cannot always effectively eradicated by the traditional approaches; however, there some alternative therapies have been presented. Honey has been used as a medicine since ancient times in many countries for its elevated antioxidant activity, as well as expressive antimicrobial activity.
Objective: the objective of this study was to investigate the effecting degree of H. pylori with different type of honey samples from Syria.
Methods: micro-dilution method and agar-well diffusion assay were carried out on H. pylori isolated from gastric biopsy. Inhibition of H. pylori urease was also tested.
Results: all honey samples inhibited the growth of H. pylori with a MIC ranging from 30-50%. Both Black seed honey and Coastal Mountain honeys at 50% (v/v%) showed a growth inhibition zone of 16 and 15 mm, respectively; being similar to the growth inhibition zone of Amoxicillin (15 mm) and Levofloxacin (14 mm). 65% of H. pylori urease activity was inhibited using Black seed honey and Coastal Mountain at 50% (v/v%).
Conclusion: These results indicate that Black seed and Coastal Mountain honey samples may be appropriate agents to treat H. pylori.
Helicobacter pylori, inhibition zone diameter, MIC, syrian honey samples, urease inhibition, colonization.
Department of Analytical and Food Chemistry, Faculty of Pharmacy, Al-Andalus University for Medical Sciences, Tartous, Department of Microbiology, Faculty of Pharmacy, Al-Andalus University for Medical Sciences, Tartous, Department of Microbiology, Faculty of Pharmacy, Al-Andalus University for Medical Sciences, Tartous