Fernando L.A. Fonseca*, Taiana C. Caetano, Carolina Z. P. Mecca, Roberta S.S. Yamaguchi, Leandro R. Bissoli, Beatriz da Costa A. Alves and Izilda A. Bagatin Pages 18 - 25 ( 8 )
Introduction: Modified quinolines have been used as metal chelators, and they have drawn the attention of researchers due to the possible biological approaches with a broad scope of application. This study aims to evaluate the in vitro biological effects of seven quinolinic compounds in RAW macrophage culture, blood cells and in Candida albicans fungus.
Methods: Cell viability and proliferation of macrophages were evaluated using trypan blue and MTT staining assays. Gene expression was analyzed through RT-qPCR. Hematological analysis was carried out using flow cytometry and coagulogram tests. The analysis of Candida albicans strains was performed through the Minimum Inhibitory Concentration test (MIC).
Results: The compounds did not significantly interfere with cell viability and proliferation of macrophages; however, compounds 6 and 7 increased the expression of both TNF-α and TGF-β1. Compound 3, in turn, only increased the expression of TNF-α and compound 4 increased TGF-β1 expression.
Conclusion: There were significant alterations in blood cellularity and in human coagulation cascade. Nevertheless, no fungicidal or fungistatic effect could be observed regarding Candida albicans.
Blood cells, Candida albicans, coagulation cascade, metallic compounds, quinoline, RAW macrophages.
Laboratorio de Analises Clinicas, Faculdade de Medicina do ABC, Av. Príncipe de Gales, 821, CEP 09060-650, Santo Andre, SP, Laboratorio de Analises Clinicas, Faculdade de Medicina do ABC, Av. Príncipe de Gales, 821, CEP 09060-650, Santo Andre, SP, Instituto de Ciencias Ambientais, Quimicas e Farmaceuticas, Laboratorio de Quimica de Calixarenos, Espectroscopia Molecular e Catalise, Universidade Federal de Sao Paulo - Rua Prof. Arthur Riedel, 275, CEP 09972-270, Diadema, SP, Centro de Terapia Celular e Molecular, Universidade Federal de Sao Paulo - Rua Mirassol, 207, CEP 04044-010, Sao Paulo, SP, Laboratorio de Analises Clinicas, Faculdade de Medicina do ABC, Av. Príncipe de Gales, 821, CEP 09060-650, Santo Andre, SP, Laboratorio de Analises Clinicas, Faculdade de Medicina do ABC, Av. Príncipe de Gales, 821, CEP 09060-650, Santo Andre, SP, Instituto de Ciencias Ambientais, Quimicas e Farmaceuticas, Laboratorio de Quimica de Calixarenos, Espectroscopia Molecular e Catalise, Universidade Federal de Sao Paulo - Rua Prof. Arthur Riedel, 275, CEP 09972-270, Diadema, SP