In vitro Evaluation of Biological Effects of Metallic Compounds with Quinolines

Author(s):

Fernando L.A. Fonseca*, Taiana C. Caetano, Carolina Z. P. Mecca, Roberta S.S. Yamaguchi, Leandro R. Bissoli, Beatriz da Costa A. Alves and Izilda A. Bagatin   Pages 18 - 25 ( 8 )

Abstract:

Introduction: Modified quinolines have been used as metal chelators, and they have drawn the attention of researchers due to the possible biological approaches with a broad scope of application. This study aims to evaluate the in vitro biological effects of seven quinolinic compounds in RAW macrophage culture, blood cells and in Candida albicans fungus.

Methods: Cell viability and proliferation of macrophages were evaluated using trypan blue and MTT staining assays. Gene expression was analyzed through RT-qPCR. Hematological analysis was carried out using flow cytometry and coagulogram tests. The analysis of Candida albicans strains was performed through the Minimum Inhibitory Concentration test (MIC).

Results: The compounds did not significantly interfere with cell viability and proliferation of macrophages; however, compounds 6 and 7 increased the expression of both TNF-α and TGF-β1. Compound 3, in turn, only increased the expression of TNF-α and compound 4 increased TGF-β1 expression.

Conclusion: There were significant alterations in blood cellularity and in human coagulation cascade. Nevertheless, no fungicidal or fungistatic effect could be observed regarding Candida albicans.

Keywords:

Blood cells, Candida albicans, coagulation cascade, metallic compounds, quinoline, RAW macrophages.

Affiliation:

Laboratorio de Analises Clinicas, Faculdade de Medicina do ABC, Av. Príncipe de Gales, 821, CEP 09060-650, Santo Andre, SP, Laboratorio de Analises Clinicas, Faculdade de Medicina do ABC, Av. Príncipe de Gales, 821, CEP 09060-650, Santo Andre, SP, Instituto de Ciencias Ambientais, Quimicas e Farmaceuticas, Laboratorio de Quimica de Calixarenos, Espectroscopia Molecular e Catalise, Universidade Federal de Sao Paulo - Rua Prof. Arthur Riedel, 275, CEP 09972-270, Diadema, SP, Centro de Terapia Celular e Molecular, Universidade Federal de Sao Paulo - Rua Mirassol, 207, CEP 04044-010, Sao Paulo, SP, Laboratorio de Analises Clinicas, Faculdade de Medicina do ABC, Av. Príncipe de Gales, 821, CEP 09060-650, Santo Andre, SP, Laboratorio de Analises Clinicas, Faculdade de Medicina do ABC, Av. Príncipe de Gales, 821, CEP 09060-650, Santo Andre, SP, Instituto de Ciencias Ambientais, Quimicas e Farmaceuticas, Laboratorio de Quimica de Calixarenos, Espectroscopia Molecular e Catalise, Universidade Federal de Sao Paulo - Rua Prof. Arthur Riedel, 275, CEP 09972-270, Diadema, SP

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